DNAsequencing相关论文
Charged single molecules of DNA can be detected and characterized with a voltage-biased solid-state nanopore immersed in......
Nanopore DNA sequencing is one of the most promising technique toward the third generation fast,low cost,and direct read......
The candidate tumor suppressor gene of HIP68 could suppress proliferation of the cancer by activatin
Cancer represents a greatest global burden on patients,families,societies.Hypoxia-inducible factors(HIFs)almost associat......
Polycystic kidney disease (PKD) includes autosomal dominant polycystic kidney disease (ADPKD) and autosomal recessive po......
Nanopore DNA sequencing is one of the most promising technique toward the third generation fast,low cost,and direct read......
Next-generation sequencing studies on cancer somatic mutations have discovered that driver mutations tend to appear in m......
A comprehensive protocol for preimplantation genetic diagnosis(PGD) for patients with hemophilia B b
Objective Hemophilia B(HB) is the second most common form of hemophilia after hemophilia A. It is estimated that one in ......
Analysis of low-density lipoprotein receptor gene mutations in a Chinese patient with clinically hom
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使用ＲＴ－ＰＣＲ方法克隆了Ｗｉｓｔａｒ大鼠脑α１型甲状腺激素受体的ｃＤＮＡ，得到包含起始及终止密码子共１２３３ｂｐ、编码４０９个氨基酸的受体全长编码离列，酶切分析后，将此特异ＤＮＡ片段......
目的性发育异常是一组多基因介导的遗传性疾病，其遗传学病因异常复杂，但临床表型的多样化，导致临床实践中诊断比较困难。近年来，基因测......
AIM: In recent years, studies have suggested that EpsteinBart virus (EBV) is associated with HCC. The present study was ......
Construction of exogenous multiple epitopes of helper T lymphocytes and DNA immunization of its chim
AIM: To design and construct an exogenous multiple epitope of helper T lymphocytes (HTL), and to evaluate its effect on ......
AIM: To study the frameshift mutations of the retinoblastomaprotein-interacting zinc finger gene RIZ in gastric cancer w......
AIM: To explore the germline mutations of the two main DNA mismatch repair genes (hMSH2and hMLH1) between patients with ......
目的 探讨聚合酶链反应-序列特异性引物(PCR-SSP)法检测人类血小板抗原(HPA)-15基因的可靠性.方法 设计序列特异性引物,采用PCR-SS......
An error-correction code (ECC) sequencing approach has recently been reported to effectively reduce sequencing errors by......
The emergence of MoS2 nanopores has provided a new avenue for high performance DNA sequencing,which is critical for mode......
Molecular phylogenetic systematics of twelve species of Acipenseriformes based on mtDNA ND4L -ND4 ge
Acipenseriformes is an endangered primitive fish group, which occupies a special place in the history of ideas concernin......
Preliminary study of single nucleotide polymorphisms of PRPS2 gene in overproducing type of gouty pa
目的:探讨编码人磷酸核糖焦磷酸合成酶亚单位2的基因PRPS2单核苷酸多态性与产生过剩型痛风患者的关系.方法:利用聚合酶链反应扩增......
提取人骨髓细胞ｍＲＮＡ，应用人ＩＬ－１１特性异引物进行ＲＴ－ＰＣＲ，将全部ＲＴ－ＰＣＲ产物以ＴＡ克隆的方式克隆人ＰＣＲ＾ＴＭ１质粒。......
从中国汉族人胎肝组织提取ＲＮＡ，以ＲＴ－ＰＣＲ方法获得了编码含号顺序的全长甘露聚糖结合凝集素肽链的ｃＤＮＡ片段，将其与ｐＧＥＭ－Ｔ载体连接，转化大肠杆菌ＴＧ１，......
利用ＰＣＲ和ＤＮＡ重组技术，成功的扩增了牛布氏菌５４４Ａ基因。回收含Ｂｒ．ａｂｏｒｔｕｓ５４４ＡＤＮＡ片段，插入ＳｍａＩ单酶切的ｐＵＣ９载体中，进行核苷酸序列分析，证实克隆的ＤＮＡ片段长度为２２４ｂｐ，在该序列中含有......
